• Preparation of prokaryotic expression systems carrying desired gene sequences encoding peptides or proteins
• Selection of laboratory-scale culture conditions regarding: type of culture medium, culture duration, and temperature to achieve the highest production yield
• Obtaining stable vectors in bacterial strains
• Scaling up the production of recombinant peptides or proteins to a pilot scale

• Gene design: identification of DNA sequences encoding the VL and VH regions of an antibody. Designing an appropriate linker between them
• Gene synthesis and cloning: insertion of the designed gene into an expression vector in bacterial systems
• Protein expression: production in the selected expression system, optimizing conditions for high yield and correct folding.
• Purification: isolation using chromatographic techniques, such as affinity chromatography, to obtain pure and functional protein

CAR construct design – development of a chimeric antigen receptor tailored to specific target antigens.

• Designing precise RNA guides tailored to specific genetic sequences and research objectives
• Preparation of vectors with a selected CRISPR/Cas system (e.g., Cas9, Cas12a), appropriately optimized for efficiency and specificity of action

• Project analysis: We discuss the client's research goals and the specifics of the samples (e.g., DNA type, sample source)
• Platform selection: We help choose the best sequencing platform tailored to your needs
• Library type: We determine the type of library suitable for your project, e.g., whole-genome sequencing (WGS), targeted sequencing, metagenomics, or RNA-seq

Programming metabolic pathways to enable the efficient production of biosimilar substances by preparing genetic constructs that encode the genes responsible for producing the enzymes that control the metabolic pathways leading to the formation of biosimilar substances

• Preparation of prokaryotic expression systems carrying desired gene sequences encoding biopolymers
• Obtaining stable vectors in bacterial strains
• Optimization of laboratory-scale culture conditions regarding: type of culture medium, culture duration, and temperature selection to achieve the highest biopolymer production yield
• Scale-up of biopolymer production to a pilot-plant scale

• DNA isolation and purification
• Checking plasmid stability in bacterial strains
• Site-directed mutagenesis reactions
• Gene design and cloning into expression vectors
• Gateway cloning

• Protein purification using low-pressure liquid chromatography (LPLC) methods to a minimum purity of 70%
• Refolding of recombinant proteins and peptides
• Purification using medium-pressure chromatography methods on a laboratory scale to a minimum purity of 90%
• Confirmation of proper tertiary structure and protein mass using MS/MS methods
• Scale-up of recombinant peptide or protein production to a pilot scale