Genetic engineering services

Recombinant pepetides and proteins

  • Construction of prokaryotic expression systems with gene sequences encoding peptides or proteins
  • Optimization of laboratory-scale culture conditions, including the selection of culture medium, cultivation time, and temperature, to maximize recombinant protein or peptide production efficiency.
  • Obtaining stable vectors in E.coli bacterial strains
  • Scale-up of recombinant peptide or protein production to the quarter-technical scale, ensuring process efficiency and consistency in expression and yield.

Recombinant antibodies (e.g. scFv, for conjugation and others)

  • Gene design: identify the DNA sequences encoding the VL and VH regions of the antibody. Designing the appropriate linker.
  • Gene synthesis and cloning: inserting the designed gene into an expression vector in bacterial systems.
  • Protein expression: production in the selected expression system, optimizing conditions for high yield and correct folding.
  • Purification: isolation by chromatographic techniques such as affinity chromatography to obtain a pure and functional protein.

CAR-T

CAR construct design - development of a chimeric antigen receptor tailored to specific target antigens

CRISPR

  • Design of precise RNA-guids tailored to specific genetic sequences and research goals.
  • Preparation of vectors with the selected CRISPR/Cas system (e.g. Cas9, Cas12a), properly optimized for efficiency and specificity of action.

Preparation of DNA library for NGS

  • Project analysis: We discuss the client's research goals and the specifics of the samples (e.g., DNA type, sample source).
  • Platform selection: We help you select the best sequencing platform tailored to your needs.
  • Library type: We determine the type of library suitable for your project, such as whole-genome sequencing (WGS), targeted sequencing, metagenomics, or RNA-seq.

Biosimilars

Engineering metabolic pathways for efficient biosimilar production by designing and constructing genetic systems encoding enzymes that regulate key metabolic processes involved in biosimilar synthesis.

Biopolymers

  • Construction of prokaryotic expression systems with sequences of genes encoding biopolymers
  • Obtaining stable vectors in E.coli bacterial strains
  • Optimization of laboratory-scale culture conditions, including the selection of culture medium, cultivation time, and temperature, to maximize recombinant protein or peptide production efficiency.
  • Scale-up of recombinant peptide or protein production to the quarter-technical scale, ensuring process efficiency and consistency in expression and yield.

DNA Services

  • DNA isolation and purification
  • Checking plasmid stability in bacterial strains
  • Site-directed mutagenesis reactions of genes
  • Designing and cloning genes into expression vectors
  • Gateway cloning

Protein Services

  • Purification of proteins by low-pressure LPLC chromatography methods to a purity of min. 70%
  • Refolding of recombinant proteins and peptides, 
  • Purification by laboratory-scale medium-pressure chromatography methods to a purity of min. 90%
  • Confirmation of correct tertiary structure and protein mass by MS/MS methods
  • Scale up production of recombinant peptides or proteins to quarter-technical scale.