• Construction of prokaryotic expression systems with gene sequences encoding peptides or proteins
• Optimization of laboratory-scale culture conditions, including the selection of culture medium, cultivation time, and temperature, to maximize recombinant protein or peptide production efficiency.
• Obtaining stable vectors in E.coli bacterial strains
• Scale-up of recombinant peptide or protein production to the quarter-technical scale, ensuring process efficiency and consistency in expression and yield.

• Gene design: identify the DNA sequences encoding the VL and VH regions of the antibody. Designing the appropriate linker.
• Gene synthesis and cloning: inserting the designed gene into an expression vector in bacterial systems.
• Protein expression: production in the selected expression system, optimizing conditions for high yield and correct folding.
• Purification: isolation by chromatographic techniques such as affinity chromatography to obtain a pure and functional protein.

CAR construct design - development of a chimeric antigen receptor tailored to specific target antigens

• Design of precise RNA-guids tailored to specific genetic sequences and research goals.
• Preparation of vectors with the selected CRISPR/Cas system (e.g. Cas9, Cas12a), properly optimized for efficiency and specificity of action.

• Project analysis: We discuss the client's research goals and the specifics of the samples (e.g., DNA type, sample source).
• Platform selection: We help you select the best sequencing platform tailored to your needs.
• Library type: We determine the type of library suitable for your project, such as whole-genome sequencing (WGS), targeted sequencing, metagenomics, or RNA-seq.

Engineering metabolic pathways for efficient biosimilar production by designing and constructing genetic systems encoding enzymes that regulate key metabolic processes involved in biosimilar synthesis.

• Construction of prokaryotic expression systems with sequences of genes encoding biopolymers
• Obtaining stable vectors in E.coli bacterial strains
• Optimization of laboratory-scale culture conditions, including the selection of culture medium, cultivation time, and temperature, to maximize recombinant protein or peptide production efficiency.
• Scale-up of recombinant peptide or protein production to the quarter-technical scale, ensuring process efficiency and consistency in expression and yield.

• DNA isolation and purification
• Checking plasmid stability in bacterial strains
• Site-directed mutagenesis reactions of genes
• Designing and cloning genes into expression vectors
• Gateway cloning

• Purification of proteins by low-pressure LPLC chromatography methods to a purity of min. 70%
• Refolding of recombinant proteins and peptides, 
• Purification by laboratory-scale medium-pressure chromatography methods to a purity of min. 90%
• Confirmation of correct tertiary structure and protein mass by MS/MS methods
• Scale up production of recombinant peptides or proteins to quarter-technical scale.